T7 Turbo RNA Polymerase (200 U/μl, GMP Grade)

Product Description:

T7 Turbo RNA polymerase (T7 RNAP), a protein encoded by the bacteriophage T7 DNA expressed in the recombinant E.coli, Clone number D05, is a DNA-dependent 5'→ 3' RNA polymerase that highly specifically recognizes T7 promoter sequences. This product uses single-stranded DNA (ssDNA) or double-stranded DNA (dsDNA) containing T7 promoter sequences as the template and NTPs as the substrate to synthesize RNA complementary to the ssDNA or dsDNA template strand downstream of the promoter. Compared with wild-type T7 RNA Polymerase, T7 Turbo RNA Polymerase effectively reduces the content of dsRNA, a by-product produced during transcription.While its co-transcription reaction product also has a higher capping rate.

Features:

1. Reducing dsRNA content more than 5-fold, significantly lowering immunogenicity.

2. Reduce cap analogue inputs by more than 75%.

3. No significant difference in yield and integrity compared with the wild type.