FastPure Plasmid Mini Kit-DC201
Cost-effective small scale preparation of plasmid
Product Description
FastPure Plasmid Mini Kit purifies 1 - 5 ml of cultured bacteria and lyses the cells by an optimized SDS-alkaline method. The kit utilizes a silica-based membrane technology in the form of a convenient spin column, and binds plasmid DNA in a highly efficient manner under high-salt and low-pH conditions and largely remove proteins, genomic DNA, RNA, and other impurities. In the end, pure plasmid DNA is eluted from the silica membrane with a lowsalt, high-pH elution buffer. The kit recovers up to 35 µg of high-copy plasmid DNA per isolation procedure.The purified plasmid DNA is ready for use in biological experiments such as enzyme digestion, PCR, sequencing, ligation, transformation, and transfection of common passage cells.
Features
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Fast and Easy: No need for column equilibration; simple and fast extraction from multiple samples within 30 min
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High Purity: Extracted pure palsmid DNA that can be used in most molecular applications (sequencing, cloning, PCR)
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High Yields: Each column adsorbs up to 35 µg of plasmid DNA
Components
Storage
Store at 15 ~ 25°C and transport at room temperature.
If ambient temperatures often exceed 25°C, we suggest storing RNase A at 2 ~ 8°C.
After addition of RNase A, Buffer P1 is stable for 6 months when stored at 2 ~ 8°C.
Features
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High Yields
The FastPure Plasmid Mini Kit was used to extract plasmids of 3.0 kb, 5.4 kb, 7.0 kb, and 10.0 kb, respectively, and the extraction products were analyzed by agarose gel electrophoresis. Results show that the kit performs well and gives high yields for plasmids of various sizes.
- High Integrity
GFP plasmid and M3K plasmid transfered to E. coli was purified by Vazyme #DC201 and similar products (Suppliers A, B, C, D and E). Plasmid DNA integrity and RNA residue were tested by 1.0% agarose gel electrophoresis. As shown in the figure, Vazyme #DC201 is equivalent to suppliers A - E. Under the same conditions, there is no RNA residue.
- Downstream Applications
The above plasmids extracted of 5.4 kb and 10.0 kb were for single-enzyme digestion and analyzed by agarose gel electrophoresis. The results demonstrate that the extracted plasmid DNA is of high purity and does not inhibit downstream reactions.
Product Manual
For Research Use Only. Not for use in diagnostic procedures.