FastPure Gel DNA Extraction Mini Kit-DC301
Gel recovery and crude DNA recovery by one kit
Product Description
This kit adopts optimized buffer system and silica gel column purification technology, which can recover 70 bp - 20 kb DNA fragments from various concentrations of TAE or TBE agarose gel. DNA adsorption column can specially adsorp DNA under high-salt condition. In addition, the kit can directly purify DNA fragments from PCR products, enzymatic reaction systems or crude DNA products obtained by other methods, and remove impurities such as proteins, other organic compounds, inorganic salt ions and oligonucleotide primers. It can ensure that the purification can be completed within 10 - 15 min. The purified DNA can be used directly for ligation, transformation, enzyme digestion, in vitro transcription, PCR, sequencing, microinjection, etc.
Features
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One kit for two uses: Applicable to the recovery of DNA from gel and purification of crude DNA products
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Simple and fast: Just add 1× volume of gel solubilization buffer; no need for column equilibration; simple and fast purification in 10 - 15 min
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Highly efficient recovery: Up to 80% recovery efficiency
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Wide recovery range of DNA fragments: Compatible with DNA fragments of 70 bp - 20 kb
Components
Storage
Store at 15 ~ 25°C and transport at room temperature.
Performance
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High Recovery Efficiency
PCR products of 50 bp, 486 bp, 1 kb, 2 kb, 4.5 kb, 7.5 kb, 10 kb, and 12 kb are purified, respectively, and both the pre-recovery and post-recovery DNA fragments were analyzed by agarose gel electrophoresis. Results show that the kit delivers good recovery in the fragment range of 50 bp - 12 kb.
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Higher Yields
Vazyme #DC301 and commercially available products (A, B, C, D, E, F, G, H) were used to purify and recover PCR products (500 bp) and enzyme digestion products (2 kb) according to their respective procedures, and the concentration were detected. The results show that the purification effect of Vazyme #DC301 is superior to other products.
Product Manual