DNase I, RNase-free (50 U/μl)-EN402

Product Description  

Deoxyribonuclease I (DNase I) is an endonuclease that digests single- or double-stranded DNA. It recognizes and cleaves phosphodiester bonds to produce a single deoxynucleotide or single- or double-stranded oligodeoxynucleotide with 5'-phosphorylated and 3'-hydroxylated ends. The activity of DNase I depends on Ca²⁺, and DNase I can also be activated by divalent metal ions such as Mg²⁺ and Mn²⁺. In the presence of Mg²⁺, DNase I nonspecifically recognizes and cleaves a double-stranded DNA at any site on either strand, and in the presence of Mn²⁺, it recognizes and cleaves almost the same sites on both strands of the DNA to produce DNA fragments with blunt ends or sticky ends with one or two nucleotide overhangs.

 Applications  

It is applicable for RNA extraction, in vitro transcription, DNA removal in RT-PCR, DNase I footprinting, nick translation, DNA library preparation and other molecular biological experiments.

 Components