2 × Phanta UniFi Master Mix-P516

Product Description  

Phanta UniFi DNA Polymerase is a next-generation high-fidelity DNA polymerase carefully selected based on the BioSmart platform, featuring ultra-high fidelity and universal primer annealing. Combined with the latest hot-start technology and superior buffer system, this product has high success rates and specificity at specific universal annealing temperatures, enabling stable amplification of various targets. Its fidelity is 220-fold higher than that of Taq polymerase, providing an efficient and convenient solution for PCR experiments requiring highly precise amplification, such as molecular cloning, sequencing, and site-directed mutagenesis. 2 × Phanta UniFi Master Mix is a premix that contains Phanta UniFi DNA Polymerase, dNTPs, Mg²⁺ , and other necessary components for PCR amplification. It only requires the addition of primers and templates for amplification, thereby reducing pipetting operations and improving detection throughput and result stability. This product is applicable for standard templates, crude products, and GC-rich systems (including primers and templates). The amplification products are blunt-ended, subsequently applicable for ClonExpress Ultra One Step Cloning Kit V3 (Vazyme #C117) and Ultra-Universal TOPO Cloning Kit (Vazyme #C603).

 Features  

• Ultra-high fidelity: ~220X higher than Taq DNA Polymerase

• Universal program: Universal primer annealing at 60°C

• Fast amplification: <10kb, 15s/kb; ≥10kb, 30s/kb

• Broad Compatibility: Suitable for complex samples, GC-rich/poor systems, and multiplex amplification

 Experimental Cases  

• Ultra-High Fidelity

  The fidelity amplification of different polymerases was determined using the next-generation sequencing method (P. Mielinis. et al. Journal of Molecular Biology (2021)). The result shows that the fidelity of Phanta UniFi DNA Polymerase is ~220 times that of Taq DNA Polymerase.