Phanta Flash Super-Fidelity DNA Polymerase-P521
Fast amplification & High fidelity
Product Description
Phanta Flash Super-Fidelity DNA Polymerase has the characteristics of high fidelity (mismatch rate is 1/106 of conventional Taq DNA polymerase) and rapid amplification (5 sec/kb), by the directed evolution of Phanta DNA Polymerase. With the application of the latest hot-start technology and the addition of specific factors, its amplification specificity and success rate are significantly improved. The amplification products of this product are blunt-ended, which are subsequently applicable for ClonExpress Ultra One Step Cloning Kit V2 (Vazyme #C116) and Ultra-Universal TOPO Cloning Kit (Vazyme #C603).
Features
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Flash amplification: Amplification of fragments within 10 kb can be completed in 1 hour
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High fidelity: ~106X higher than Taq DNA Polymerase
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Broad compatibility: Suitable for crude templates, high GC/AT templates (overall GC 24%-79%, local GC 22%-88%) and methylated templates
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Convenient operation: Only ''standalone enzyme + buffer'', makes sample addition easier
Experimental Cases
▲ P510 is the master mix format of P521. P520 is the Dye Plus format of P521.
Components
Storage
Store at -30 ~ -15°C and transport at ≤0°C.
Product Manual
For Research Use Only. Not for use in diagnostic procedures.