ClonExpress Ultra One Step Cloning KitV-C116

Product Description  

The ClonExpress technology is simple, fast, and highly efficient DNA seamless cloning technology. It enables rapid directional cloning of inserts into any site in any vector. Use any method to linearize the vector, and introduce the end sequence of the linearized vector at the 5' end of the insert forward/reverse amplification primer, so that the 5' and 3' ends of the PCR product have the same ends sequence (15 - 20 bp) as the linearized vector, respectively. The PCR product with the same sequence as the end of the vector and the linearized vector are mixed in a certain proportion. Under the catalysis of recombinase, the transformation can be performed at 50°C for 5 - 30 min to complete the directional cloning.

ClonExpress Ultra One Step Cloning Kit V2 is a new generation of recombinant cloning kits, compatible with 1 - 5 fragments homologous recombination. Highly optimized 2 × CE Mix further significantly improves the recombination efficiency of multiple fragments. This product has broader compatibility with the GC content of homology arms, which better guarantees the success rate of cloning in more difficult application scenarios.

 Features  

• Simple: Ready-to-use super mix in one tube

• Fast: Recombination only takes 5 - 30 minutes

• Efficient: Efficiently clone 50 bp - 10 kb fragments, the positive rate is > 95%

• Compatible: Suitable for GC-rich/AT-rich homologous sequences

• Special optimization: Maximum compatible with 15 - 35 bp overlaps, not only 15 - 20 bp

 Experimental Cases  

• Simple and Fast Workflow: It is recommended to use Vazyme's online primer design software - CE Design for designing primers. The kit uses the properties of a proprietary ''Exnase'' Recombinase to assemble DNA fragments with shared terminal end homology without leaving any extra sequences or scars behind (seamless cloning). Transform the assembled product into competent cells directly, and hundreds of single clones will be formed on the plate for positive screening.