T7 RNA Polymerase Variants Toolbox-DD4126

 Product Description  

All four T7 RNA Polymerase, variant proteins encoded by the bacteriophage T7 DNA expressed in the recombinant E.coli, are DNA-dependent 5'→3'RNA polymerase that highly specifically recognizes T7 promoter sequences. These enzymes use single-stranded DNA(ssDNA) or double stranded DNA(dsDNA) containing T7 promoter sequences as the template and NTPs as he substrate to synthesize RNA complementary to the DNA template strand downstream of the promoter.

Compared to the Wild-Type T7 RNA polymerase:

T7 High-Capping RNA Polymerase (C10)

• Enhances co-transcriptional capping efficiency

• Adapting GG-Initiation(+1,+2) DNA template for co-transcriptional capping 

T7 High-Integrity RNA Polymerase (P02)

• Improve the integrity of in vitro transcription products by 3-5%  

T7 Thermostable RNA Polymerase (M1)

Maintains catalytic activity at 50℃

• Suitable for circRNA synthesis with group I intron self-splicng and reduces dsRNA via high-temperature IVT

T7 Low-dsRNA RNA Polymerase (D13)

• Reduce dsRNA byproducts to 5% compared to WT counterpart

  Features  

 C10: 

• Cap analog Input ↓ ≥95%, utilizing wild type T7 promoter (GG)

• Reduces R&D costs

   D13: 

• dsRNA ↓ ~100× vs wild-type

• Meets USP/EMA dsRNA limits

• Reduces immune activation risks

  P02: 

• Integrity ↑ 10% for mRNAs >8 kb

• Enables complex therapeutics (e.g., saRNA, CRISPR, CGT)

   

  M01: 

• Half-life >5 hours at 50°C

• Supports continuous manufacturing

• Suitable for circRNA synthesis

 Performance  

• C10 High-Capping