Firefly Luciferase mRNA-DD4511
Firefly Luciferase stock mRNA, suitable for experiments in mRNA delivery, translation efficiency, cell viability, and in vivo imaging.
Product Description
Firefly Luciferase mRNA enters the cell and expresses the firefly luciferase protein, which catalyzes the oxidation of D-luciferase in an ATP-dependent manner, producing fluorescence at 560nm. Originally extracted from Firefly Photinus pyralis, Firefly Luciferase is commonly used as a bioluminescent reporter gene for gene regulation and function studies, suitable for experiments such as mRNA delivery, translation efficiency, cell viability, and in vivo imaging.
This mRNA contains high level of Cap1 structure at 5' terminal.Compared with the Cap 0 structure, the mRNA with the Cap 1 structure is more suitable for the mammalian system and has a higher transcription efficiency. Modified UTP and addition of poly(A) tails provide higher transcriptional efficiency and inhibit RNA-mediated innate immune activation while enhancing mRNA stability and longevity in vitro and in vivo.
Features
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Ready-to-use & Time-saving: Eliminates time-consuming mRNA preparation—ready for immediate transfection or LNP formulation.
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High Capping Efficiency: Features Cap 1 structure, optimized for enhanced compatibility in mammalian systems.
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Incorporation of Modified Nucleotides and poly(A) Tail: Mitigates RNA-induced innate immune activation and improves mRNA stability.
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High Transfection Efficiency & Excellent Reproducibility: Ensures consistent and reliable experimental results.
Performance
mRNA cell expression process
Firefly Luciferase mRNA transfection efficiency in HEK 293T cells 20 hours after transfection
mRNA expression process in mice
Liposome encapsulated mRNA-Luc model mouse in vivo 60 min imaging detection
Components
Storage
Store at -30~-15℃ and transport at ≤0℃.
For Research Use Only. Not for use in diagnostic procedures.