Add&Read Human IL6 Quantitative Detection Kit(Customized)-DD2703-C

 Product Description  

Interleukin-6 (IL6) is a pleiotropic cytokine mainly produced by macrophages, T lymphocytes, and B  lymphocytes. It regulates the growth, differentiation, immune responses, acute-phase reactions,  and hematopoietic functions of various cells, and plays an important role in the body's immune  response to infections. IL6 plays a central role in acute inflammatory reactions, and serum IL6 levels  can increase in various infectious diseases. IL6 activation has a short duration, quick decline during  recovery, and a large magnitude, making it an excellent indicator for assessing early inflammation,  sepsis, infection severity, and prognosis. This reagent kit utilizes a sandwich method to detect IL6  levels. The kit contains IL6 Standard and two monoclonal antibodies specific to IL6, one coupled  with Eu (donor, Anti-IL6-Eu) and the other coupled with A3 (acceptor, Anti-IL6-A3). When both  antibodies bind to IL6 simultaneously, the proximity of Anti-IL6-Eu and Anti-IL6-A3 allows for fluorescence resonance energy transfer (FRET) to occur. Use 320/340 nm excitation light to excite the  fluorescent donor, which emits 620 nm light. The 620 nm light excites the fluorescent receptor,  which emits 665 nm light. The IL6 concentration in the sample is proportional to the FRET signal  (ratio of fluorescence intensity at 665 nm to 620 nm).

 Features  

• Easy to operate: just add sample and incubate at room temperature and read plate

• Save sample: only 16μL is needed

• Fast: saving 80% time compared with ELISA

• High-throughput: suitable for automated instruments

 Storage  

The reagent kit should be stored at -30 ~ -15℃ and transported at ≤0℃