Add＆Read Human IFN gamma Quantitative Detection Kit-DD2706

 Product Description  

Interferon-gamma (IFN gamma) is a pro-inflammatory cytokine primarily produced by T cells and  natural killer (NK) cells, playing a crucial role in antiviral defense mechanisms within the body.  IFN-gamma assembles into soluble dimers intracellularly and is secreted into the extracellular  space. It is involved in antiviral defense, activation of macrophages, enhancement of neutrophil and  monocyte function, and upregulation of MHC-I and MHC-II expression in cells.  This reagent kit utilizes a sandwich method to detect IFN gamma levels. The kit contains IFN  gamma Standard and two monoclonal antibodies specific to IFN gamma, one coupled with Eu  (donor, Anti-IFN gamma-Eu) and the other coupled with A3 (acceptor, Anti-IFN gamma-A3). When  both antibodies bind to IFN gamma simultaneously, the proximity of Anti-IFN gamma-Eu and  Anti-IFN gamma-A3 allows for fluorescence resonance energy transfer (FRET) to occur. Use  320/340 nm excitation light to excite the fluorescent donor, which emits 620 nm light. The 620 nm  light excites the fluorescent receptor, which emits 665 nm light. The IFN gamma concentration in the  sample is proportional to the FRET signal (ratio of fluorescence intensity at 665 nm to 620 nm).

 Features  

• Easy to operate: just add sample and incubate at room temperature and read plate

• Save sample: only 16μL is needed

• Fast: saving 80% time compared with ELISA

• High-throughput: suitable for automated instruments

 Storage  

The reagent kit should be stored at -30 ~ -15℃ and transported at ≤0℃