Add＆Read Human IL7 Quantitative Detection Kit-DD2711

 Product Description  

Interleukin-7 (IL-7) is a pleiotropic cytokine primarily produced by bone marrow stromal cells. Its main functions include promoting the growth of B and T cells and exerting anti-apoptotic effect s.  It plays a crucial role in the growth, survival, and differentiation of T cells, while also exhibiting  potential chemotactic activity. Like other cytokines, IL-7 is an immunoregulatory protein that plays an important role in the normal development of the immune system and the maintenance of  normal immune function. This kit uses a sandwich assay to detect IL-7 levels. The kit includes IL-7 Standard and two  monoclonal antibodies specifically recognizing IL-7. One antibody is conjugated with Eu (donor,  Anti-IL-7-Eu), and the other is conjugated with A3 (acceptor, Anti-IL-7-A3). When both antibodies bind to IL-7 simultaneously, Anti-IL-7-Eu and Anti-IL-7-A3 come into close proximity, enabling  fluorescence resonance energy transfer (FRET). The fluorescence donor is excited by light at  320/340 nm, emitting light at 620 nm. This 620 nm light then excites the fluorescence acceptor,  which emits light at 665 nm. The concentration of IL-7 in the sample is proportional to the FRET signal value (the ratio of light intensity at 665 nm to 620 nm).

 Features  

• Easy to operate: just add sample and incubate at room temperature and read plate

• Save sample: only 16μL is needed

• Fast: saving 80% time compared with ELISA

• High-throughput: suitable for automated instruments

 Storage  

The reagent kit should be stored at -30 ~ -15℃ and transported at ≤0℃